Congo Red Stain Kit
Intended use:
Congo red stain is intended for staining amyloid in tissue.
Principle:
Amyloid substance has a selective affinity to Congo red and can be stained readily. Congo red, which is an azo dye with linear configuration, binds hydroxyl of amyloid substance with its amino group while clings on fiber of amyloid substance in parallel and appears red.
Methods:
1. Deparaffinize according to a typical procedure and hydrate tissue in water.
2. Stain in Congo red solution for 10-20 minutes.
3. Differentiate directly in alkaline alcohol solution for several seconds.
4. Rinse in distilled water for 5 minutes.
5. Stain nuclei in Mayer's Hematoxylin for 2 minutes. Rinse in distilled water for 5 minutes.
6. Dehydrate and clear in the typical manner. Mount with mounting media.
Specifications:
|
Contents |
3vialsx20ml/kit |
3Btlsx100ml/kit |
Components |
|
Congo red solution |
20ml |
100ml |
Congo red, Methanol |
|
Alkaline alcohol solution |
20ml |
100ml |
Potassium hydroxide |
|
Mayer hematoxylin solution |
20ml |
100ml |
Hematoxylin |
Precaution:
- Both amyloid substance and elastin fiber will be stained by the Congo red but appear in different shapes, so distinguish the two carefully.
- Differentiate appropriately in alkaline alcohol solution. If not enough, collagen fiber will also stain; if excessive, amyloid will discolor.
- The unstained amyloid paraffin tissue will gradually lose the affinity to Congo red after one year of storage.
Expected Results:
|
Amyloid, erythrocyte, elastin fibre |
—— |
Red |
|
Nuclei |
—— |
Blue |
|
Background |
—— |
Yellowy pink |